Fumaric acid overproduction in yeast mutants deficient in fumarase
نویسندگان
چکیده
منابع مشابه
Mutants of Yeast Deficient in Cytochrome C.
YTOCHROME C is an ideal protein for biochemical studies because of its ease of isolation and low molecular weight. Its primary structure from a number of species is entirely (cf. SMITH, MATSUBARA, MCDOWALL and ROTHFUS 1963) or partially (cf. TUPPY 1958) known. Therefore, any system exhibiting genetic control of cytochrome c would be of considerable interest for investigating geneprotein relatio...
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Yeast cells almost completely deficient in all cytochromes were obtained by introducing two defective nuclear genes, cyd1 and cyc4, into the same haploid strain. The action of the two mutant genes is synergistic, since either gene acting singly results in only partial cytochrome deficiency. Normal synthesis of all cytochromes can be restored in the double mutant by adding delta-aminolevulinic a...
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Fumarase C is responsible for the reversible hydration/dehydration of S-malate to fumarate during the catabolic Kreb’s cycle. Using PCR based site-directed mutagenesis; two active site mutants of fumarase C have been constructed. The recombinant enzymes, S139A and S140A, were purified using metal affinity chromatography and subjected to kinetic analysis and X-ray crystallization. The kinetic in...
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Fumaric acid esters (FAE) are chemical compounds derived from the unsaturated dicarbonic acid fumaric acid. The usage of FAEs in treatment of psoriasis was introduced in the late 1950's. In the 1980s more standardized oral preparations of FAEs were developed containing dimethylfumarate (DMF) and salts of monoethylfumarate (MEF) as main compounds. In 1994, Fumaderm an enteric-coated tablet conta...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1992
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1992.tb05192.x